Dissecting Maxillary Processes from E11.5 Mouse Embryos
4:12
Isolating Whole Cell Protein Lysates from Mouse Maxillary Processes
5:06
Isolating Whole Cell Protein Lysates from Primary and/or Immortalized Mouse Embryonic Palatal Mesenchyme Cells
6:22
Results: Assessing Phosphorylated Protein Levels in Cell Lysates from Cultured Mouse Embryonic Palatal Mesenchyme Cells
6:59
Conclusion
필기록
This protocol can be used to gain greater insight into the dynamics and roles of phosphoproteins active during mammalian craniofacial development. This protocol overcomes the common barrier of dephosphorylation during protein isolation from two ph
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The protocol presents a method for isolating whole cell protein lysates from dissected mouse embryo facial processes or cultured mouse embryonic palatal mesenchyme cells and performing subsequent western blotting to assess phosphorylated protein levels.