JoVE Logo
Faculty Resource Center

Sign In

Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model

DOI :

10.3791/63949-v

August 8th, 2022

August 8th, 2022

2,459 Views

1Department of Cardiovascular Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine, 3Department of Cardiovascular Surgery, The First Affiliated Hospital, Jinan University, 4Guangdong Provincial Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, 5Department of Anesthesiology, Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine

Based on the familial hereditary cardiomyopathy family found in our clinical work, we created a C57BL/6N mouse model with a point mutation (G823E) at the mouse MYH7 locus through CRISPR/Cas9-mediated genome engineering to verify this mutation.

-- Views

Related Videos

article

Detection of Copy Number Alterations Using Single Cell Sequencing

article

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

article

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

article

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

article

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

article

Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

article

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

article

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models

article

Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique

article

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved