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Capturing Chromosome Conformation Across Length Scales

DOI :

10.3791/64001-v

10:15 min

January 20th, 2023

January 20th, 2023

2,546 Views

1Department of Systems Biology, University of Massachusetts Medical School, 2Howard Hughes Medical Institute

Hi-C 3.0 is an improved Hi-C protocol that combines formaldehyde and disuccinimidyl glutarate crosslinkers with a cocktail of DpnII and DdeI restriction enzymes to increase the signal-to-noise ratio and the resolution of chromatin interaction detection.

Tags

Chromosome Conformation

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