Isolation of Mediastinal Lymph Nodes, Lungs, Mesenteric Lymph Node Chains, and Small Intestine
2:37
Preparation of Single-Cell Suspensions from the Small Intestine, Lung, and Lymph Nodes
6:20
Results: Flow Cytometry Analysis and Gating Strategy for the Identification of Th9 Cells and IL-9+ ILC2 Cells
7:48
Conclusion
Transcript
This protocol allows for the isolation and characterization of lymphoid cells expressing IL-9 in N.brasiliensis non-lymphoid infected tissues including the infected cut which recently was technically invisible.This protocol also provides an altern
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IL-9-expressing T and ILC2 cells are induced during N. brasiliensis infection, yet their characterization has been largely overlooked in the infected intestine due to their low frequency and differential kinetics. This protocol describes the isolation of these cells from different target organs and confirmation of their identity via flow cytometry at different infection stages.