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Gene Trapping Using Gal4 in Zebrafish

DOI :

10.3791/50113-v

13:34 min

September 29th, 2013

September 29th, 2013

17,654 Views

1Department of Biology, Temple University

This protocol describes the method of gene trap insertional mutagenesis using Gal4-VP16 as the primary reporter and GFP/RFP as secondary reporters in zebrafish. Approximately one in ten high-expressing F0 fish yield gene trap progeny co-expressing GFP and RFP. The screening procedure can be readily scaled to adapt to the size of the laboratory performing the insertional mutagenesis screen.

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Gene Trapping

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