Preparation of Fatty Acid Methyl Esters (FAMEs) and GCMS Analysis
4:08
Deuterium Acetone Exchange of Plasma Samples to Determine Body Water Enrichment
5:04
Results: Plasma Body Water Enrichment from D2O, and Brown Adipose Tissue Total Fatty Acid Enrichment and Synthesis after 3 Days of D2O Administration
6:34
Conclusion
Transcript
Contrary to other metabolic pathways like glucose disposal, fatty acid synthesis is not routinely assessed, leading to incomplete interpretations of the metabolic status. The key benefits of this method is that it's relatively non-invasive, and it
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Here, we present an inexpensive quantitative method utilizing deuterium oxide and gas chromatography mass spectrometry (GCMS) for the analysis of total fatty acid de novo lipogenesis in brown adipose tissue in vivo.