Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
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13:11 min
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February 10th, 2023
DOI :
February 10th, 2023
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Chapters in this video
0:04
Introduction
0:51
Expression of ncAA‐Bearing Proteins in Salmonella
2:22
In Vitro Fluorescence Labeling of ncAA‐Bearing Proteins in Salmonella Typhimurium
3:27
Bio‐Orthogonal Labeling of Salmonella Secreted Effector SseJ‐F10TCO‐HA in HeLa Cells
8:08
Super‐Resolution (dSTORM) Imaging
9:57
Results: Incorporation of TCO*A into SseJ‐F10TCO‐HA Expressed in Escherichia coli and Salmonella, and Super‐Resolution Imaging of GCE‐Labeled Secreted SseJ‐J10TCO‐HA in HeLa Cells
12:27
Conclusion
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