Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis
4.6K Views
•
12:41 min
•
December 23rd, 2022
DOI :
December 23rd, 2022
•Explore More Videos
Chapters in this video
0:05
Introduction
0:53
Day 0: Preparation of the 2- or 3-Day-Old Organoids
2:55
Days 2 - 3: Harvesting the 2- or 3-Day-Old Organoids
4:16
Seeding the 2- or 3-Day-Old Organoids Using a Pipetting Robot
5:27
Day 4: Drug Treatment and Reagent Dispensing with Digital Drug Dispenser
7:49
Image Acquisition with the Live-Cell Imager and Analysis of Data
10:12
Results: The Single-Organoid Dose-Response Analysis
12:03
Conclusion
Related Videos
Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology
10.8K Views
Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
11.9K Views
Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening
8.5K Views
Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion
10.1K Views
Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
10.8K Views
Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array (HMCA)-based Plates
9.0K Views
A Simple Migration/Invasion Workflow Using an Automated Live-cell Imager
7.6K Views
Establishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids
6.4K Views
A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
9.8K Views
Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish
7.9K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved