A High-Throughput Platform for Culture and 3D Imaging of Organoids

2.4K Views

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07:42 min

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October 14th, 2022

DOI :

10.3791/64405-v

October 14th, 2022

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Gianluca Grenci¹^,², Florian Dilasser¹, Saburnisha Binte Mohamad Raffi¹, Marion Marchand¹, Mona Suryana¹, Geetika Sahni¹, Virgile Viasnoff¹^,³^,⁴, Anne Beghin¹^,⁵

¹Mechanobiology Institute (MBI), National University of Singapore, ²Biomedical Engineering Department, National University of Singapore, ³Department of Biological Sciences, National University of Singapore, ⁴IRL 3639 CNRS, ⁵Department of Microbiology & Immunology, Immunology Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore

Summary

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High throughput Platform

Organoids

Microtexture Cell Culture

Laboratory Procedures

Chapters in this video

0:04

Introduction

0:49

PDMS Mold Dicing and Production of the UV‐Curable, Adhesive, Textured Layer

2:19

Coverslip Substrate Preparation

3:34

Transferring the Textured Film to the Final Substrate

4:33

Cell Seeding, Release, and Collection of the Organoids

6:29

Results: Fabrication of the Microwell Culture Dish for a High‐Density Organoid Culture and Differentiation

7:00

Conclusion

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