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Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow

DOI :

10.3791/64707-v

9:03 min

March 17th, 2023

March 17th, 2023

857 Views

1Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A STAR), 2Thermo Fisher Scientific, 3Thermo Fisher Scientific

Here, we present a protocol to harvest adherent cells from multi-layered flasks in a closed semi-automated manner using a counterflow centrifugation system. This protocol can be applied for harvesting both adherent and suspension cells from other cell expansion platforms with few modifications to the existing steps.

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Keywords Mesenchymal Stem Cells

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