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Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow

DOI :

10.3791/64707-v

9:03 min

March 17th, 2023

March 17th, 2023

632 Views

1Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A STAR), 2Thermo Fisher Scientific, 3Thermo Fisher Scientific

Here, we present a protocol to harvest adherent cells from multi-layered flasks in a closed semi-automated manner using a counterflow centrifugation system. This protocol can be applied for harvesting both adherent and suspension cells from other cell expansion platforms with few modifications to the existing steps.

Tags

Mesenchymal Stem Cell Processing

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