Results: Analyzing the Different Fluorescence Image Datasets
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Conclusion
Transcript
This method quantifies the spatiotemporal dynamics of polarized cells along its midline expressing fluorescent reporter or dye for specific entity of interest such as actin, ions, or vesicle dynamics. The version of automation eliminates biases an
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Current methods for analyzing the intracellular dynamics of polarized single cells are often manual and lack standardization. This manuscript introduces a novel image analysis pipeline for automating midline extraction of single polarized cells and quantifying spatiotemporal behavior from time lapses in a user-friendly online interface.