Light-Induced Dielectrophoresis for Characterizing the Electrical Behavior of Human Mesenchymal Stem Cells

1.6K Views

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10:08 min

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June 16th, 2023

DOI :

10.3791/64909-v

June 16th, 2023

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Kiara L. Lacy¹, Samuel Salib¹, Mary Tran², Tunglin Tsai¹, Rominna Valentine¹, Herdeline Ann M. Ardoña¹^,²^,³^,⁴, Tayloria N. G. Adams¹^,²^,⁴

¹Department of Chemical and Biomolecular Engineering, Samueli School of Engineering, University of California, Irvine, ²Department of Biomedical Engineering, Samueli School of Engineering, University of California, Irvine, ³Department of Chemistry, School of Physical Sciences, University of California, Irvine, ⁴Sue & Bill Gross Stem Cell Research Center, University of California, Irvine

Summary

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Light Induced Dielectrophoresis

LiDEP

Human Mesenchymal Stem Cells

Electrical Behavior

Stem Cell Heterogeneity

Microfluidic Device

Virtual Electrode Configuration

ITO Glass Slide

Microchannel Preparation

Copper Tape Application

Experimental Procedure

Zuri Rashad

Chapters in this video

0:05

Introduction

0:50

Light-Induced Dielectrophoresis (LiDEP) Chip Device Fabrication

3:05

Dielectrophoresis (DEP) Buffer Preparation

3:55

Cell Preparation

4:40

Light-Induced Dielectrophoresis (LiDEP) Characterization

7:41

Results: Dielectrophoresis Responses in hMSCs

9:28

Conclusion

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