Dual-Dye Optical Mapping of Hearts from RyR2^R2474S Knock-In Mice of Catecholaminergic Polymorphic Ventricular Tachycardia

1.0K Views

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09:36 min

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December 22nd, 2023

DOI :

10.3791/65082-v

December 22nd, 2023

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Yangpeng Li*¹^,², Jun Yang*¹, Rui Zhang¹, Tangting Chen¹, Shiyu Zhang¹, Yuqing Zheng¹, Qiang Wen³, Tao Li¹, Xiaoqiu Tan¹^,², Ming Lei¹^,⁴, Xianhong Ou¹

¹Key Laboratory of Medical Electrophysiology of Ministry of Education, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Institute of Cardiovascular Research, Southwest Medical University, ²Department of Cardiology, the Affiliated Hospital of Southwest Medical University, ³Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, ⁴Department of Pharmacology, University of Oxford

Summary

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Dual Dye Optical Mapping

RyR2R2474S Knock In Mice

Catecholaminergic Polymorphic Ventricular Tachycardia

Electrophysiological Properties

Ventricular Arrest

Membrane Potential

Intercellular Calcium Signals

Cardiac Arrhythmic Disease

Langendorff System

Electrocardiogram ECG

Blebbistatin CREBs Solution

Excitation contraction Uncoupling

Rhod 2 AM

Perfusion System

Contraction Artifacts

Chapters in this video

0:04

Introduction

1:00

Mouse Heart Harvest, Cannulation, and Perfusion

3:14

Optical Mapping and Arrhythmia Induction

6:38

Results: APD80 and CaTD80 Analysis at 10 Hz Before and After ISO Challenge

8:36

Conclusion

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Dual-Dye Optical Mapping of Hearts from RyR2R2474S Knock-In Mice of Catecholaminergic Polymorphic Ventricular Tachycardia

Dual-Dye Optical Mapping of Hearts from RyR2^R2474S Knock-In Mice of Catecholaminergic Polymorphic Ventricular Tachycardia