Establishment of Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts From Fresh Tissue

3.5K Views

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07:31 min

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May 26th, 2023

DOI :

10.3791/65229-v

May 26th, 2023

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¹Molecular Epidemiology and Predictive Tumor Markers Group, Area 3, Ramón y Cajal Health Research Institute (IRYCIS), ²The Biomedical Research Network in Cancer (CIBERONC), ³Biobank and Biomodels Platform (PT20/0045), ISCIII research and development platforms in biomedicine and health sciences, BioBank Hospital Ramón y Cajal-IRYCIS, Spanish National Biobanks Network (ISCIII Biobank Register No. B.0000678), Ramón y Cajal Health Research Institute (IRYCIS), ⁴Faculty of Medicine, University of Alcalá de Henares, ⁵Institute of Tissue Medicine and Pathology, University of Bern, ⁶Department of Molecular Oncology, Cancer Research Institute, Biomedical Research Center of the Slovak Academy of Sciences, ⁷Experimental Oncology, Molecular Oncology Program, Centro Nacional de Investigaciones Oncológicas (CNIO), ⁸Department of Surgical Oncology, National Cancer Institute, Slovak Medical University, ⁹Pancreatic and Biliopancreatic Surgery Unit, Hospital Universitario Ramón y Cajal, ¹⁰Department of Pathology, Hospital Universitario Ramón y Cajal, ¹¹Department of Radiation Oncology, Hospital Universitario Ramón y Cajal, ¹²Department of Cancer, Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), ¹³Cancer Stem Cell and Fibroinflammatory Group, Chronic Diseases and Cancer, Area 3, IRYCIS

Summary

Explore More Videos

Pancreatic Cancer

Tumor Organoids

Fibroblasts

Translational Research

Personalized Medicine

In Vitro Models

Spatial Transcriptomics

Patient derived

Preclinical Models

Standardized Protocol

Treatment Sensitivity

Tumor Microenvironment

Three dimensional Models

Cell Culture Techniques

Extracellular Environment

Chapters in this video

0:00

Introduction

1:59

Fresh Primary Tumor Tissue Processing to Establish Tumor Organoids and Primary Fibroblasts

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