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Establishment and Culture of Patient-Derived Breast Organoids

DOI :

10.3791/64889-v

February 17th, 2023

February 17th, 2023

3,059 Views

1Cold Spring Harbor Laboratory, Cold Spring Harbor, 2Genetics Graduate Program, Stony Brook University

A detailed protocol is provided here for establishing human breast organoids from patient-derived breast tumor resections or normal breast tissue. The protocol provides comprehensive step-by-step instructions for culturing, freezing, and thawing human patient-derived breast organoids.

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