JoVE Logo
Faculty Resource Center

Sign In

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

DOI :

10.3791/4449-v

November 5th, 2012

November 5th, 2012

16,449 Views

1Department of Biology, University of North Carolina at Charlotte

Xenopus egg extract is a useful model system to investigate the DNA damage checkpoint. This protocol is for the preparation of Xenopus egg extracts and DNA damage checkpoint inducing reagents. These techniques are adaptable to a variety of DNA damaging approaches in the study of the DNA damage checkpoint signaling.

Tags

DNA Damage Checkpoint

-- Views

Related Videos

article

Testing Visual Sensitivity to the Speed and Direction of Motion in Lizards

article

Computer-Generated Animal Model Stimuli

article

Preparation and Fractionation of Xenopus laevis Egg Extracts

article

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

article

Fertilization of Xenopus oocytes using the Host Transfer Method

article

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

article

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

article

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

article

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

article

Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved