In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

7.7K Views

•

10:00 min

•

November 28th, 2019

DOI :

10.3791/60551-v

November 28th, 2019

•

Daniela Avila-González¹, Guadalupe García-López¹, Néstor E. Díaz-Martínez², Héctor Flores-Herrera³, Anayansi Molina-Hernández¹, Wendy Portillo⁴, Néstor F. Díaz¹

¹Departamento de Fisiología y Desarrollo Celular, Instituto Nacional de Perinatología, ²Biotecnología Médica y Farmacéutica CONACYT, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), ³Departamento de Inmunobioquímica, Instituto Nacional de Perinatología, ⁴Instituto de Neurobiología, UNAM

Summary

Explore More Videos

In Vitro Culture

Epithelial Cells

Human Amniotic Membrane

Anatomical Regions

Cell Heterogeneity

Functional Properties

Chapters in this video

0:04

Title

0:43

Mechanical Amniotic Membrane Separation

2:40

Membrane Washing

3:36

Enzymatic Amniotic Membrane Digestion

6:21

Human Amniotic Epithelial Cells (HAEC) Isolation and Culture

7:31

Results: Representative HEAC Morphologic and Phenotypic Analyses

9:12

Conclusion

Related Videos

11:53

Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos

8.8K Views

06:02

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

12.1K Views

12:21

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol

10.5K Views

08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

6.9K Views

08:37

Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization

23.9K Views

14:37

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

10.9K Views

08:21

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

8.5K Views

09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

9.2K Views

10:48

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells

13.2K Views

07:38

Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis

8.3K Views