Optical Clearing and Labeling for Light-sheet Fluorescence Microscopy in Large-scale Human Brain Imaging
1.8K Views
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06:52 min
•
January 26th, 2024
DOI :
January 26th, 2024
•Danila Di Meo*1, Josephine Ramazzotti*1, Marina Scardigli*1,2, Franco Cheli1, Luca Pesce1,3, Niamh Brady1, Giacomo Mazzamuto1,4,5, Irene Costantini1,4,6, Francesco S. Pavone1,4,5
1European Laboratory for Non-linear Spectroscopy (LENS), University of Florence, 2Division of Physiology, Department of Experimental and Clinical Medicine, University of Florence, 3Department of Physics, University of Pisa, 4National Research Council - National Institute of Optics (CNR-INO), 5Department of Physics and Astronomy, University of Florence, 6Department of Biology, University of Florence
Chapters in this video
0:00
Introduction
1:18
Preparing Formalin‐Fixed Human Brain Sections for SHORT Tissue Transformation Technique
2:01
SWITCH ‐ H2O2 ‐ Antigen Retrieval — TDE (SHORT) Tissue Transformation Technique
4:55
Sample Mounting for Light‐Sheet Fluorescence Microscopy and Stripping for Multi‐Round Labeling of the Same Specimens
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