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Imaging and Quantification of Intact Neuronal Dendrites via CLARITY Tissue Clearing

DOI :

10.3791/62532-v

April 20th, 2021

April 20th, 2021

2,817 Views

1Department of Genetics and Genomics, Baylor College of Medicine, 2Medical Scientist Training Program, Baylor College of Medicine, 3Department of Neuroscience, Baylor College of Medicine

Neuronal dendritic morphology often underlies function. Indeed, many disease processes that affect the development of neurons manifest with a morphological phenotype. This protocol describes a simple and powerful method for analyzing intact dendritic arbors and their associated spines.

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