Optical Clearing and Labeling for Light-sheet Fluorescence Microscopy in Large-scale Human Brain Imaging

1.7K Views

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06:52 min

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January 26th, 2024

DOI :

10.3791/65960-v

January 26th, 2024

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Danila Di Meo*¹, Josephine Ramazzotti*¹, Marina Scardigli*¹^,², Franco Cheli¹, Luca Pesce¹^,³, Niamh Brady¹, Giacomo Mazzamuto¹^,⁴^,⁵, Irene Costantini¹^,⁴^,⁶, Francesco S. Pavone¹^,⁴^,⁵

¹European Laboratory for Non-linear Spectroscopy (LENS), University of Florence, ²Division of Physiology, Department of Experimental and Clinical Medicine, University of Florence, ³Department of Physics, University of Pisa, ⁴National Research Council - National Institute of Optics (CNR-INO), ⁵Department of Physics and Astronomy, University of Florence, ⁶Department of Biology, University of Florence

Transcript

Summary

Explore More Videos

Optical Clearing

Light sheet Fluorescence Microscopy

Human Brain Imaging

3D Reconstruction

Cytoarchitecture

Tissue Transformation

SHORT Protocol

Neuronal Markers

Multi labeling

Autofluorescent Signals

High throughput Protocol

Postmortem Processing

Subcellular Resolution

Chapters in this video

0:00

Introduction

1:18

Preparing Formalin‐Fixed Human Brain Sections for SHORT Tissue Transformation Technique

2:01

SWITCH ‐ H₂O₂ ‐ Antigen Retrieval — TDE (SHORT) Tissue Transformation Technique

4:55

Sample Mounting for Light‐Sheet Fluorescence Microscopy and Stripping for Multi‐Round Labeling of the Same Specimens

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