JoVE Logo

Sign In

A Hydrophobic Tissue Clearing Method for Rat Brain Tissue

3.9K Views

08:24 min

December 23rd, 2020

DOI :

10.3791/61821-v

December 23rd, 2020


Explore More Videos

Hydrophobic Tissue Clearing

Chapters in this video

0:05

Introduction

0:46

Sample Preparation

1:36

Dehydration and Depigmentation

3:04

CTB-Cholera Toxin Subunit B Labeling and Antibody Application

4:17

Tissue Clearing

5:02

Mounting the Sample for Imaging

5:59

Results: Confocal Images of the Hydrophobically Cleared Tissue Samples with Fluorescent Labelled TH Neurons and CTB Markers

7:39

Conclusion

Related Videos

article

09:29

2-Vessel Occlusion/Hypotension: A Rat Model of Global Brain Ischemia

19.8K Views

article

10:21

Metabolic Support of Excised, Living Brain Tissues During Magnetic Resonance Microscopy Acquisition

6.0K Views

article

08:36

Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry

10.7K Views

article

05:12

A Micro-CT-based Method for Characterizing Lesions and Locating Electrodes in Small Animal Brains

8.4K Views

article

06:33

Massive Pontine Hemorrhage by Dual Injection of Autologous Blood

4.1K Views

article

07:45

Imaging and Quantification of Intact Neuronal Dendrites via CLARITY Tissue Clearing

3.4K Views

article

05:17

Investigation of Spatial Interaction Between Astrocytes and Neurons in Cleared Brains

2.4K Views

article

06:53

Extraction and Tissue Clearing Preparation of Mouse Brain-Spinal Cord Samples

100 Views

article

01:19

A Hydrophobic Tissue Clearing Method for a Rat Brain Tissue Sample

24 Views

article

01:28

A Method for the Isolation of Neurovascular Units from Rat Brain

59 Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved