需要订阅 JoVE 才能查看此. 登录或开始免费试用。
A Porcine Model of Acute Respiratory Failure with a Continuous Infusion of Oleic Acid
In This Article
Summary
Infusing oleic acid continuously into the pulmonary artery of an anesthetized adult pig induces acute respiratory failure, enabling controlled experimentation during acute respiratory decompensation.
Abstract
This protocol outlines an acute respiratory distress model utilizing centrally administered oleic acid infusion in Yorkshire pigs. Prior to experimentation, each pig underwent general anesthesia, endotracheal intubation, and mechanical ventilation, and was equipped with bilateral jugular vein central vascular access catheters. Oleic acid was administered through a dedicated pulmonary artery catheter at a rate of 0.2 mL/kg/h. The infusion lasted for 60-120 min, inducing respiratory distress. Throughout the experiment, various parameters including heart rate, respiratory rate, arterial blood pressure, central venous pressure, pulmonary artery pressure, pulmonary capillary wedge pressure, end-tidal carbon dioxide, peak airway pressures, and plateau pressures were monitored. Around the 60 min mark, decreases in partial arterial oxygen pressure (PaO2) and fraction of oxygen-saturated hemoglobin (SpO2) were observed. Periodic hemodynamic instability, accompanied by acute increases in pulmonary artery pressures, occurred during the infusion. Post-infusion, histological analysis of the lung parenchyma revealed changes indicative of parenchymal damage and acute disease processes, confirming the effectiveness of the model in simulating acute respiratory decompensation.
Introduction
The utilization of porcine models in translational research holds significant importance in advancing our understanding of human medicine1. Porcine models, due to their physiological and anatomical similarities to humans, provide a valuable platform for studying complex diseases and therapeutic interventions1. In the context of acute respiratory failure, porcine models offer a unique opportunity to investigate the pathophysiological mechanisms, evaluate treatment strategies, and assess potential interventions1,2,3. The ability t....
Protocol
The protocol received approval from the Vanderbilt University Institutional Animal Care and Use Committee (protocol M1800176-00) and strictly adhered to the National Institute of Health Guidelines for the Care and Use of Laboratory Animals. Male and female Yorkshire pigs, weighing approximately 40-45 kg, were utilized in this experiment. The animals were obtained from a commercial source (see Table of Materials). The current practice does not involve screening for any pre-existing medical conditions in t.......
Representative Results
Early single pig, pilot data demonstrates an increase in RIVA-RI prior to changes in other respiratory monitoring measures (RR and SpO2), in line with changes in PaO2 (Figure 3). The drop in PaO2 is the "positive" result this model intends to achieve. Preliminary data also shows that RIVA-RI increases and the PaO2 decreases with disease progression starting at the 30-min mark (Figure 3; red arrow). PaO
Discussion
The key element in this protocol is to closely monitor the hemodynamic condition of the pig during the administration of oleic acid to induce respiratory distress15. It is of the utmost importance for researchers to take the necessary time to appropriately position the hemodynamic monitoring devices. One specific drawback of this model is the potential hemodynamic instability that may arise as a result of inflammation and injury to the respiratory vasculature8,
Acknowledgements
The authors would like to thank Dr. José A. Diaz, Jamie Adcock, Mary Susan Fultz and the S.R. Light Laboratory at Vanderbilt University Medical Center for their assistance and support. This work was supported by a grant from the National Heart, Lung, and Blood Institute of the National Institutes of Health (BA; R01HL148244). The content is the sole responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
....Materials
| Name | Company | Catalog Number | Comments |
| 1% Isoflurane | Primal, Boston, MA, USA | 26675-46-7 | https://www.sigmaaldrich.com/US/en/product/aldrich/792632?gclid=Cj0KCQjw9fqnBhDSARIsAHl cQYS_W-q6tS2s6LQw2Qn7Roa3T GIpTLPf52351vrhgp44foEcRozPqt YaAtvfEALw_wcB |
| Arterial Catheter | Merit Medical, South Jordan, UT, USA | MAK401 | MAK Mini Access Kit 4F |
| Blood Pressure Amp | AD Instruments, Colorado Springs, CO, USA | FE117 | https://www.adinstruments.com/products/bp-blood-pressure-amp |
| Central Venous Catheter | Arrow International, Cleveland, OH, USA | AK-09800 | 8.5 Fr. x 4" (10 cm) Arrow-Flex |
| Disposable Pressure Transducers | AD Instruments, Colorado Springs, CO, USA | MLT0670 | https://www.adinstruments.com/products/disposable-bp-transducers |
| Edwards Lifesciences Triple Stage Venous Cannulas | Edwards Life Sciences, Irvine, CA | TF293702 | https://www.graylinemedical.com/products/edwards-lifesciences-triple-stage-venous-cannulas-venous-dual-stage-cannula-tf293702?variant=31851942576185&gad=1& gclid=Cj0KCQiAr8eqBhD3ARIsAIe -buNdmkzavUBaIx-1be7boWn2kW hbUR6QCjaobB08uuK9qJW66JvY TM4aAufGEALw_wcB |
| Kelly Scissors | MPM Medical Supply, Freehold, NJ 07728 | 104-5516 | https://www.mpmmedicalsupply.com/products/kelly-scissors |
| Kendall 930 FoamElectrodes | Covidien, Mansfield, MA, USA | 22935 | https://www.cardinalhealth.com/en/product-solutions/medical/patient-monitoring/electrocardiography/monitoring-ecg-electrodes/radiolucent-electrodes/kendall-930-series-radiolucent-foam-electrodes.html |
| Ketamine Hydrochloride 100 mg/mL, Injectable Solution, 10 mL | Patterson Veterinary, Loveland, CO 80538 | 07-894-8462 | https://www.pattersonvet.com/ProductItem/078948462?omni=ketamine |
| LabChart 8 software | AD Instruments, Colorado Springs, CO, USA | N/A | https://www.adinstruments.com/products/labchart |
| Lahey Retractor | BOSS Instruments LTD, Gordonsville, VA 22942 | 18-1210 | https://bossinstruments.com/product/7-3-4-lahey-thyroid-retractor-6mmx28mm/ |
| Oleic Acid | Sigma-Aldrich, Merck, Darmstadt, Germany | O1008 | https://www.sigmaaldrich.com/US/en/product/sial/o1008?gclid=CjwKCAjwzJmlBhBBEiwAEJy Lu2047wRpXqF_Z2BegUyhgZJ _WygsWfErhgrGCIyMp8PxwNH sTZ8qARoCl1QQAvD_BwE&gcl src=aw.ds |
| Peripheral IV Catheter Angiocath 18-24 G 1.16 inch | McKesson, Irving, TX, USA | 329830 | https://mms.mckesson.com/product/329830/Becton-Dickinson-381144 |
| Piezoelectrode | MuRata Manuractoring Co, Ltd., Nagaokakyo, Kyoto, Japan | 7BB-12-9 | https://www.murata.com/en-us/products/productdetail?partno=7BB-12-9 |
| PlasmaLyte | Baxter International, Deerfield, IL, USA | 2B2544X | https://www.ciamedical.com/baxter-2b2544x-each-solution-plasma-lyte-a-inj-ph-7-4-1000ml |
| Pulmonary Artery Catheter | Edwards Life Sciences, Irvine, CA | 131F7 | Swan Ganz 7F x 110cm |
| Standard Endotracheal Tubes | Teleflex, Morrisville, NC 27560 | 5-10313 | https://www.teleflex.com/usa/en/product-areas/anesthesia/airway-management/endotracheal-tubes/standard-tubes/index.html |
| SurgiVet Clearview Foley Catheter, 8 Fr, 55 cm Silicone | Penn Veterinary Supply, Inc, West Rendering, PN 13971 | SVCFC1030 | https://www.pennvet.com/customer/portal/catalog/home?urile=wcm:path%3APennVet+Catalog/Super+Sku+Catalog/SS0672/Surgivet+Clearview+Silicone+Foley+Catheters |
| Telazol (Tiletamine HCl and Zolazepam HCl), Injectable Solution, 5 mL | Patterson Veterinary, Loveland, CO 80538 | 07-801-4969 | https://www.pattersonvet.com/ProductItem/078014969?omni=telazol |
| Welch Allyn E-MacIntosh Standard Laryngoscope Blade | MFIMedical, San Diego, CA 92131 | WLA-69242 | https://mfimedical.com/products/welch-allyn-e-macintosh-standard-laryngoscope-blade?variant=12965771870285¤cy =USD&utm_medium=product_sync &utm_source=google&utm_content =sag_organic&utm_campaign=sag _organic&gclid=Cj0KCQiAr8eqBhD 3ARIsAIe-buMhpgM96qRXkCUKA 6Mhmdat0p93JbecCGTaLStexhV pkUVa9VkWUzgaAr-iEALw_wcB |
| Xylazine HCl 100 mg/mL, Injectable Solution, 50 mL | Patterson Veterinary, Loveland, CO 80538 | 07-894-5244 | https://www.pattersonvet.com/ProductItem/078945244 |
| Yorkshire Pigs | Oak Hill Genetics, Ewing, IL, USA | 138274 | Female/Male Swine- Yorkshire/Landrace 81-100lbs |
References
- Judge, E. P., et al. Anatomy and bronchoscopy of the porcine lung. A model for translational respiratory medicine. Am J Respir Cell Mol Biol. 51 (3), 334-343 (2014).
- Anna Bassols, C. P., et al.
This article has been published
Video Coming Soon
关于 JoVE
版权所属 © 2025 MyJoVE 公司版权所有，本公司不涉及任何医疗业务和医疗服务。