Preparation and Embedding of Live Zebrafish Embryos for Imaging
3:54
5D Confocal Imaging of Live Zebrafish Embryos
7:10
Results: Mitotic Cell Division Imaging in Zebrafish Embryos
8:43
Conclusion
Transcript
The overall goal of this procedure is to monitor the fidelity of chromosome segregation during mitosis in a live zebrafish embryo by using flourescently labeled chromatin and cell-membrane proteins that will be captured using high-resolution confo
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Mitosis is critical to every living organism and defects often lead to cancer and developmental disorders. Using this imaging protocol and zebrafish as a model system, researchers can visualize mitosis in a live vertebrate organism and the multitude of defects that arise when mitotic processes are defective.
