Depleting Macrophages using Clodronate-containing Liposomes
2:15
Derivation of Polarized Macrophages from Bone Marrow Aspirates
4:24
Transferring Macrophages into Mice
5:17
Results: Clodronate Liposome Depletion of Macrophages Allows Adoptive Transfer of Experimental Macrophage Populations
8:23
Conclusion
Macrophages play a central role in homeostasis and pathology in many tissues. The protocol presented here describes methods for depleting macrophages in vivo, deriving polarized macrophages from bone marrow aspirates, and adoptively transferring macrophages into mice. These techniques allow determination of the role that polarized macrophages play in health and disease.
