Results: Electronic Pre-Resonance Stimulated Raman Scattering Imaging of Raman Dyes
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Conclusion
副本
Highly-multiplexed vibrational imaging provides a one-shot optical approach to interrogate multiple protein markers in tissues with subcellular resolution. This platform provides a comprehensive picture of protein interactions of biological system
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Electronic pre-resonance stimulated Raman scattering (epr-SRS) imaging of rainbow-like Raman dyes is a new platform for highly multiplexed epitope-based protein imaging. Here, we present a practical guide including antibody preparation, tissue sample staining, SRS microscope assembly, and epr-SRS tissue imaging.