February 10th, 2023
•Presented here is a protocol to initiate, maintain, and analyze mouse hematopoietic stem cell cultures using ex vivo polyvinyl alcohol-based expansion, as well as methods to genetically manipulate them by lentiviral transduction and electroporation.
Tags
Related Videos
Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
Method for Culture of Early Chick Embryos ex vivo (New Culture)
Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures
Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy
Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates