2.4K Views
•
07:55 min
•
February 10th, 2023
DOI :
February 10th, 2023
•Chapters in this video
0:05
Introduction
1:05
Bone Marrow Extraction and c-Kit+ Enrichment of Hematopoietic Stem Cell (HSC)
3:03
Initiating Cell Cultures with c-Kit-Enriched Hematopoietic Stem and Progenitor Cells (HSPCs)
3:45
Performing Media Changes
4:19
Electroporating Cultured HSPCs
5:24
Transducing Cultured HSPCs with Lentiviral Vector
6:17
Results: Fluorescence-Activated Cell Sorting (FACS) of the c-Kit-Enriched HSCs
7:22
Conclusion
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