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Methods for the Study of the Zebrafish Maxillary Barbel

12.3K Views

09:30 min

November 23rd, 2009

November 23rd, 2009

12,337 Views

0:00

Title

0:42

Introduction

1:07

Barbel Development

1:47

Barbel Clipping

4:38

Agar Embedding of Matched Barbel Pairs

9:08

Conclusion

The zebrafish maxillary barbel is an integumentary sense organ containing ectodermal, mesodermal and neural crest derivatives. Importantly, the adult barbel can regenerate after proximal amputation. This video introduces maxillary barbel development and demonstrates a surgical protocol to induce regeneration, followed by collection, embedding and downstream imaging of barbel specimens.

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