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12.1K Views
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17:59 min
April 3rd, 2011
DOI :
10.3791/2598-v
Kapitel
0:01
Title
0:05
Introduction
0:34
Dissection of Tumor
4:11
Culturing Lymphoma Cells
10:33
Feeding the Cells
11:36
Passaging the Cells
11:44
Initial Passage
15:17
Maintaining Established Cell Lines
16:42
Freezing and Recovering the Cell Lines
17:33
Cell Recovery from Liquid Nitrogen
Zusammenfassung
In this video we demonstrate a protocol to establish mouse thymic lymphoma cell lines. By following this protocol, we have successfully established several T-cell lines from Atm-/- and p53-/- mice with thymic lymphoma.
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