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JoVE Journal

Immunology and Infection

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A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

14.4K Views

16:04 min

June 19th, 2013

June 19th, 2013

14,422 Views

0:05

Title

1:59

Generating the GPRS Packaging Cell Line: A Tet-dependent SVGmu Cell Line

5:33

Constructing DC-LV Producer Cells by Concatemer Array Transfection

9:14

Evaluating Viral Production of Each Cell Clone

10:54

Producing and Concentrating Lentiviral Vectors

12:16

Results: Isolation of High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

15:15

Conclusion

Here, we use retroviral transduction and concatemeric transfection to create a cell line that can express the components of a lentiviral vector (LV) in the absence of tetracycline. This LV encodes GFP and is pseudotyped with a glycoprotein, SVGmu, which is specific for a receptor on dendritic cells.

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