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09:44 min
March 3rd, 2015
DOI :
10.3791/52568-v
Kapitel
0:05
Title
1:30
Day 1: Prepare and Seed Cells
2:44
Day 2: Treat the Cells with Library Compounds
4:19
Day 3: Luciferase Assay
5:27
Counter-screening
7:09
Results: Modulators of the 3' UTR of MYCN
9:11
Conclusion
Summary
Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.
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