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JoVE Journal

Developmental Biology

This content is Open Access.

Long-term Live Imaging of Drosophila Eye Disc

8.2K Views

06:08 min

May 6th, 2017

May 6th, 2017

8,243 Views

0:05

Title

1:04

Disc Dissection

2:43

Mounting

4:04

Confocal Microscopy

4:48

Results: Sustained Photoreceptor Differentiation during 10 h Live Imaging

5:44

Conclusion

Transkript

This protocol describes a detailed method for the long-term ex vivo culture and live imaging of a Drosophila imaginal disc. It demonstrates photoreceptor differentiation and ommatidial rotation within the 10 h period of live imaging of the eye disc. The protocol is simple and does not require expensive setup.

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