Isolation of the Mesenteric Lymph Nodes, Intestines, and Peyer's Patches
3:45
Isolation of Lymphocytes from the Inductive Sites
4:22
Isolation of Intraepithelial Lymphocytes from the Small Intestine
7:28
Isolation of Lamina Propria Lymphocytes from the Small Intestine
9:39
Results: Characterization of Isolated Lymphocytes
10:52
Conclusion
Transkript
The overall goal of this protocol is to isolate lymphocytes from intestinal inductive sites including Peyer's patches and mesenteric lymph nodes and effector sites including lamina propria and epithelium. This method can help answer key questions
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Here we describe a detailed protocol for the isolation of lymphocytes from the inductive sites including the gut-associated lymphoid tissue Peyer's patches and the draining mesenteric lymph nodes, and the effector sites including the lamina propria and the intestinal epithelium of the small intestinal immune system.