Anmelden

JoVE Journal

Immunology and Infection

Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.

Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus

6.2K Views

10:09 min

November 6th, 2018

November 6th, 2018

6,177 Views

0:04

Title

0:43

Sample Preparation and Fixation

1:44

Precoating of the Coverslip with Polystyrene Beads and Coating of Poly-L-lysine onto the Bead-coated Coverslip

3:08

Immobilization of Cells on the Coverslip and Permeabilization of Cyanobacterial Cells

4:23

Photobleaching of the Chlorophyll Pigments in a Blocking Step

4:51

Antibody Binding

6:07

Image Acquisition of STORM Data

7:51

Results: Super-resolution Imaging of the FtsZ Ringin in the Cyanobacterium Prochlorococcus

9:42

Conclusion

Transkript

This method can help answer key questions on protein localization and the dynamics in photosynthetic organisms. By combining the photobleaching method with super-resolution microscope, we are able to detect the protein dynamics of photosynthetic o

Melden Sie sich an oder starten Sie Ihre kostenlose Testversion, um auf diese Inhalt zuzugreifen

Here we describe a photobleaching method to reduce the autofluorescence of cyanobacteria. After photobleaching, stochastic optical reconstruction microscopy is used to obtain three-dimensional super-resolution images of the cyanobacterial FtsZ ring.

Wir verwenden Cookies, um Ihre Erfahrung auf unserer Website zu verbessern.

Indem Sie unsere Website weiterhin nutzen oder auf „Weiter“ klicken, stimmen Sie zu, unsere Cookies zu akzeptieren.