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JoVE Journal

Cancer Research

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Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

7.7K Views

06:32 min

January 9th, 2019

January 9th, 2019

7,740 Views

0:04

Title

0:38

Fixation of Tumor NS

1:21

Paraffin Embedding of Tumor NS

1:51

Sectioning of Paraffin-Embedded NS

3:23

Immunohistochemistry (IHC) of Paraffin-Embedded NS

5:31

Results: NS Generated, Analyzed via Bioluminescence, and Stained for Glioma Biomarkers

6:10

Conclusion

Transkript

This protocol is significant because can be used to study the presence of glioma biomarker by a method that preserves the 3D structure of tumor neurospheres. The main advantage of this protocol is that the morphology of neurospheres is maintained

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Neurospheres grown as 3D cultures constitute a powerful tool to study glioma biology. Here we present a protocol to perform immunohistochemistry while maintaining the 3D structure of glioma neurospheres through paraffin embedding. This method enables the characterization of glioma neurosphere properties such as stemness and neural differentiation.

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