In Vitro-Propagated VX2 Cell Suspension Preparation
2:35
In Vivo-Propagated VX2 Cell Suspension Preparation
3:27
Surgical Field Preparation
4:17
Laparotomy and Uterine Horn Identification
5:31
Myometrial VX2 Cell Inoculation
6:21
Surgical Incision Closure and Post-Operative Care
8:03
Results: VX2 Tumor Establishment and Characterization
9:09
Conclusion
Transkript
This VX2 endometrial cancer model with retroperitoneal lymph node metastasis can be used to study technology for image-guided surgery. The main advantages of this technique are that it is reliable, simple, and that it create consistent metastatic
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This protocol presents a standardized method to grow VX2 cells in culture and to create an orthotopic VX2 model of endometrial cancer with retroperitoneal lymph node metastases in rabbits. Orthotopic endometrial cancer models are important for the pre-clinical study of novel imaging modalities for the diagnosis of lymph node metastases.