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Intracellular Refolding Assay

14.0K Views

07:18 min

January 24th, 2012

January 24th, 2012

14,038 Views

0:05

Title

1:12

Seeding the Cells

2:08

Transfection

3:02

Heat Shock

3:47

Cell Lysis and Luciferase Assay

6:17

Protein Refolding Dynamics

6:52

Conclusion

In this protocol a method to measure intracellular protein refolding after heat shock is described. This method can be used to study foldases like molecular chaperones and their co-factors or compounds able to influence their activity. Firefly luciferase activity is used as reporter to measure chaperone refolding activity.

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