Results: Real-time Activation and Dose Dependent Responses
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Conclusion
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Our protocol enables to measure detection and discrimination of volatile odors with a panel of odorant receptors. This method opens up the possibility to develop odorant receptor-based biosensors. This technique allows real-time in vitro monitorin
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Physiologically, odorant receptors are activated by odorant molecules inhaled in the vapor phase. However, most in vitro systems utilize liquid phase odorant stimulation. Here, we present a method that allows real-time in vitro monitoring of odorant receptor activation upon odorant stimulation in vapor phase.