Cerebellum Dissection and Cell Suspension Preparation
2:34
Immunolabeling of Stem Cells
3:44
Magnetic Column Preparation and Cell Sorting
4:22
Passaging of Neurospheres and Differentiation
5:27
Results: Prominin-1 Stem Cells Isolated from Postnatal Cerebellum
6:15
Conclusion
Transcripción
This protocol describes an easy and cost effective method to purify stem cells from the postnatal cerebellum. These stem cells account for the late-born molecularly garbled interneurons, but they also account for postnatally-derived cerebellar ast
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Demonstrated here is an efficient and cost-effective method to purify, culture, and differentiate white matter stem cells from postnatal mouse cerebellum.