Preparation of Organoid Culture Dishes and Seeding of Cells
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Results: Self Assembled Organoids in 2D and 3D, ECM and ECM-free Culture Conditions
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Conclusion
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These protocols enable the user to produce a large number of structurally mimetic and endocrine functional testicular organoids in both ECM and DCM-free environments in 2D or 3D culture conditions. These techniques are highly reproducible, easily
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Here, four methods for generating testicular organoids from primary neonatal murine testicular cells are described i.e., extracellular matrix (ECM) and ECM-free 2D and 3D culture environments. These techniques have multiple research applications and are especially useful for studying testicular development and physiology in vitro.