This protocol isolates high quality total RNA from fecal samples of animal and human subjects. A commercial miRNA isolation kit is used with significant adaption to isolate pure RNA with optimized quantity and quality. The RNA isolates are good for most downstream RNA assays such as sequencing, micro-array, and RT-PCR.
Videos relacionados
RNA Interference in Ticks
Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
Nucleocapsid Annealing-Mediated Electrophoresis (NAME) Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors
Optimization of a Quantitative Micro-neutralization Assay
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens
Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis
Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados