Isolation and Culture of Primary Human Saphenous Vein Endothelial Cells (hSVECs)
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Applying Shear Stress on Human SVECs
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Applying Cyclic Stretch on Human SVECs
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Results: Effects of Mechanical Stress in Human SVECs
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Conclusion
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The saphenous vein is suddenly exposed to arterial conditions after coronary artery bypass surgery. Understanding its adaptation response to mechanical stress may help designing therapeutic tools to prevent vein graft failure. This endothelial cel
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We describe a protocol to isolate and culture human saphenous vein endothelial cells (hSVECs). We also provide detailed methods to produce shear stress and stretch to study mechanical stress in hSVECs.