Nous tenons à remercier notre conseiller, la Dre Eve Marder. Cette recherche a été soutenue par l&#39;Institut national de bourses en santé NS17813 à Eve Marder et NS059255 à AE T.

<ol><li> Avant la dissection du homard devrait être enterré dans la glace pendant 30 minutes à anesthésier l&#39;animal. </li><li> Le sexe du homard peut être déterminé en regardant la forme de la nageoire d&#39;abord. Les mâles ont pléopodes Boney et les femelles ont pléopodes plumeuses. <img src="/files/ftp_upload/1320/1320fig1.png" alt="" /></li><li> Isoler le céphalothorax. <ol><li> Utilisez un mouvement de torsion pour arnaquer les griffes avant. </li><li> Retirer de l&#39;abdomen (partie arrière) en utilisant les gros ciseaux pour faire une coupure profonde entre le céphalothorax et l&#39;abdomen. Cette coupe les faisceaux de fibres géantes contrôlant la réponse la queue flip. Ensuite, passez à couper le ventre. </li><li> Retirez les antennes en les taillant avec des ciseaux, et enlevez les jambes et les maxillipèdes tiers. </li></ol></li><li> Retirer la carapace de partout dans le ventre, (ie la région de la tête entre la rainure du col utérin et la tribune). <ol><li> Démarrage sur le bord latéral de cette section et l&#39;utilisation Rongeurs spatule pour retirer soigneusement la carapace dure, tout en laissant l&#39;hypoderme rougeâtre sous-jacente intacte. La spatule est utile pour séparer les couches de sorte que la carapace peut être rompu avec les rongeurs. </li><li> Déplacement vers les yeux vous trouvez bilatérale légère calcification des plaques ovales de petite taille (IIIa osselets). Aussi près que possible de ces osselets, soigneusement détacher le muscle de la chaîne des osselets de l&#39;aide de petits ciseaux. </li><li> En utilisant une spatule et retirer la carapace des rongeurs entre les yeux et la partie caudale (environ 1 centimètre) de la tribune. </li></ol></li><li> Détacher la face (région, y compris les yeux, et la tribune). <ol><li> L&#39;utilisation de petits ciseaux faire une coupe horizontale à travers le tissu le plus rostrale exposés dans la tribune pour le détacher de l&#39;exosquelette. Puis utilisez pointe de ciseaux et / ou spatule pour enrouler soigneusement le tissu caudalement, juste après le niveau de la chaîne des osselets IIIa. </li><li> À partir dorsalement, l&#39;utilisation de petits ciseaux pour détacher les tissus restants de l&#39;exosquelette visage. Coupez le plus près à la carapace dure que possible, arrêter quand vous obtenez près du centre et de l&#39;attachement de la bouche. </li><li> Du point de vue ventrale de la mandibule doit être facilement visible. Détachez maxillipèdes restantes. </li><li> Localisez la lèvre molle sous les mandibules. </li><li> Faire des coupes latérales pour détacher le visage tout en gardant la lèvre attachée à l&#39;estomac. (Il est un peu courbée partie bleuâtre bouche extérieure qui peut être utilisé comme un guide.) </li></ol></li><li> Détacher et retirer l&#39;estomac. <ol><li> Tenez le homard dans la paume de votre main, face ventrale et de telle sorte que l&#39;extrémité antérieure est vers votre coude. </li><li> Fixer une pince hémostatique à la lèvre et laisser la gravité tirer la hémostatique et la lèvre vers le bas et loin de la paroi ventrale du corps. </li><li> L&#39;utilisation de petits ciseaux couper mandibules. </li><li> Repérez la face dorsale de la paroi du corps ventrale, qui forme un disque dur &quot;plateau&quot; à la lèvre ventrale et l&#39;estomac. Coupez juste au-dessous du «plateau», très proche de la paroi du corps, à travers l&#39;ensemble de la ligne médiane médial-latéral. Tout le côté ventral de l&#39;estomac devrait maintenant être détaché de la paroi du corps. </li><li> Tournez le homard de telle sorte que la face dorsale est en hausse. Coupez le long du bord rostral de l&#39;cervicale sillon, où la carapace a été enlevée, et tire doucement sur le ventre. </li><li> Détachez toutes les intestins supplémentaires qui restent attachés à l&#39;estomac. </li></ol></li><li> Ouvrez l&#39;estomac, et le préparer pour l&#39;épinglage. <ol><li> Tenir l&#39;estomac dans la paume de votre main avec l&#39;hypoderme vers le bas. Le hémostatique doit être encore attachée à la lèvre et devrait se situer entre vos doigts. </li><li> Utilisez des ciseaux pour faire une petite superficielle antéro-postérieure coupé de l&#39;ouverture buccale (en dessous de la lèvre) vers le bas par la face ventrale du pylore. </li><li> Pour ouvrir davantage l&#39;estomac, faire deux coupes latérales le long de la chaîne des osselets qui traverse le centre de l&#39;estomac. </li><li> Une solution saline froide peut être versé dans l&#39;estomac pour améliorer la visibilité et d&#39;expulser le contenu stomacal. </li><li> Afin de permettre la préparation à plat dans le plat dissection utiliser les petits ciseaux pour couper les trois dents gastriques. </li></ol></li><li> Pin de l&#39;estomac dans le plat de dissection. <ol><li> Placez l&#39;estomac, l&#39;hypoderme vers le haut, dans un plat de dissection remplis de solution saline. </li><li> Placez broches d&#39;insectes à la lèvre et aux quatre coins créé lorsque l&#39;estomac a été ouvert. Pour les coins antérieure broches et haut lieu près du bord, pour ne pas déranger les ganglions de la commissure des nerfs ou inférieur oesophagien. Pour les broches postérieures coins lieu un peu élevée pour ne pas déranger le muscle dilatateur du pylore. </li></ol></li></ol>

<ol>
	<li>Marder, E., Bucher, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Central+pattern+generators+and+the+control+of+rhythmic+movements.">Central pattern generators and the control of rhythmic movements.</a> Curr. Biol. 11, R986-R996 (2001).</li><li>Nusbaum, M. P., Beenhakker, M. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+small-systems+approach+to+motor+pattern+generation.">A small-systems approach to motor pattern generation.</a> Nature. 417, 343-350 (2002).</li><li>Clemens, S., Massabuau, J. C., Legeay, A., Meyrand, P., Simmers, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+modulation+of+interacting+central+pattern+generators+in+lobster+stomatogastric+ganglion:+influence+of+feeding+and+partial+pressure+of+oxygen.">In vivo modulation of interacting central pattern generators in lobster stomatogastric ganglion: influence of feeding and partial pressure of oxygen.</a> J. Neurosci. 18, 2788-2799 (1998).</li><li>Tobin, A. E., Bierman, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Homarus+americanus+stomatogastric+nervous+system+dissection.">Homarus americanus stomatogastric nervous system dissection.</a> J Vis Exp. 27, (2009).</li><li>Maynard, D. M., Dando, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+structure+of+the+stomatogastric+neuromuscular+system+in+Callinectes+sapidus,+Homarus+Americanus+and+Panulirus+argus+(Decapoda+crustacean).">The structure of the stomatogastric neuromuscular system in Callinectes sapidus, Homarus Americanus and Panulirus argus (Decapoda crustacean).</a> Philos. Trans. R. Soc. Lond. B Biol. Sci. 268, 161-220 (1974).</li><li>Herrick, F. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Natural+history+of+the+American+Lobster.">Natural history of the American Lobster.</a> U.S. Bur. Fish. , 219-252 (1909).</li><li>Marder, E., Bucher, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Understanding+circuit+dynamics+using+the+stomatogastric+nervous+system+of+lobsters+and+crabs.">Understanding circuit dynamics using the stomatogastric nervous system of lobsters and crabs.</a> Annu. Rev. Physiol. 69, 291-316 (2007).</li></ol>

Dans notre laboratoire de dissection de l&#39;estomac du homard est fait pour accéder au système nerveux stomatogastric, un réseau qui contrôle les contractions rythmiques de l&#39;estomac. L&#39;anatomie de l&#39;estomac du homard a été bien décrite précédemment 5-7, et nous recommandons de se familiariser avec son anatomie. Cela permettra de réduire le risque d&#39;endommager les nerfs et les muscles délicats. À la fin de la dissection d&#39;estomac, différentes por...

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		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<tr>
<td>Homarus americanus</td>
<td>&nbsp;</td>
<td>Commercial Lobster</td>
<td>&nbsp;</td>
<td>Can be purchased from any lobster supplier, We specifically request &#x201C;chicken&#x201D; lobsters.</td>
</tr>
<tr>
<td>Black Sylgard 170</td>
<td>&nbsp;</td>
<td>Dow Corning</td>
<td>170</td>
<td>&nbsp;</td>
<tr>
<td>Preparation Dish</td>
<td>&nbsp;</td>
<td>Fischer </td>
<td>08-807</td>
<td>Fill 2" with Black Sylgard 170.</td>
</tr>
<tr>
<td>Fine Scissors</td>
<td>&nbsp;</td>
<td>FST</td>
<td>14090-09</td>
<td>&nbsp;</td>
<tr>
<td>Insect Pins size 6</td>
<td>&nbsp;</td>
<td>FST</td>
<td>26000-65</td>
<td>&nbsp;</td>
<tr>
<td>Mayo Scissors</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>This is a common product offered by many suppliers of dissecting tools.</td>
</tr>
<tr>
<td>Micro-Spatula</td>
<td>&nbsp;</td>
<td>Ward Sci.</td>
<td>15 V 4313</td>
<td>This is a very common product offered by many suppliers of lab tools.</td>
</tr>
<tr>
<td>Rongeurs</td>
<td>&nbsp;</td>
<td>FST</td>
<td>&nbsp;</td>
<td>16000-14</td>
</tr>
<tr>
<td>Plastic hemostat</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>This is a common product offered by many suppliers of dissecting or jewelry making tools.</td>
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gross dissection of the stomach of the lobster, homarus americanus

L&#39;estomac du homard d&#39;Amérique (<i&gt; Homarus americanus</i&gt;) Est situé dans le céphalothorax, entre la tribune et la rainure du col. L&#39;extrémité antérieure de l&#39;estomac est défini par l&#39;ouverture de la bouche et l&#39;extrémité postérieure par le bas du pylore. Le long de la face dorsale de l&#39;estomac se trouve le système nerveux stomatogastric (STNS). Ce système nerveux, qui contient des réseaux rythmiques qui sous-tendent le comportement alimentaire, est un système modèle établi pour l&#39;étude des réseaux du rythme de production et de neuromodulation<sup&gt; 1,2</sup&gt;. Bien qu&#39;il soit possible d&#39;étudier ce système<i&gt; In vivo</i<sup&gt; 3</sup&gt;, Le STNS continue à produire ses activités rythmiques lorsqu&#39;ils sont isolés<i&gt; In vitro</i&gt;. Afin d&#39;étudier ce système<i&gt; In vitro</i&gt; L&#39;estomac doit être retiré de l&#39;animal. Cet article décrit comment la vidéo de l&#39;estomac peut être disséqué par le homard américain. Dans un article accompagnant la vidéo<sup&gt; 4</sup&gt; Nous démontrons comment le STNS peut être isolé de l&#39;estomac.

Watch this Scientific Journal Video about Gross Dissection of the Stomach of the Lobster, Homarus Americanus at JoVE.com

Gross Dissection of the Stomach of the Lobster, Homarus Americanus

Nous décrivons la dissection brut de l&#39;estomac du homard d&#39;Amérique (<i&gt; Homarus americanus</i&gt;).

Dissection brut de l&#39;estomac du homard, Homarus americanus</em

The stomach of the American lobster (Homarus americanus) is located in the cephalothorax, between the rostrum and the cervical groove. The anterior end of ...

gross-dissection-of-the-stomach-of-the-lobster-homarus-americanus

Research

JoVE Journal

Biology

Gross Dissection of the Stomach of the Lobster, Homarus Americanus

14.5K vues.  Brandeis. Nous décrivons la dissection brut de l'estomac du homard d'Amérique ( Homarus americanus).

Vidéo: Gross Dissection of the Stomach of the Lobster, Homarus Americanus

Dissection of Drosophila Ovaries

Dissection des ovaires chez la drosophile

Recherche

Biologie

Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region

Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates micro-dissection of four major brain regions include olfactory bulb, frontal cortex, striatum and hippocampus in fresh rat brain tissue. Useful tips for quick removal of respective regions to avoid RNA and protein degradation of the tissue are given.

Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates micro-dissection of four major brain regions include olfactory bulb, frontal cortex, striatum and hippocampus in fresh rat brain tissue. Useful tips for quick removal of respective regions to avoid RNA and protein degradation of the tissue are given.

Micro-dissection du cerveau de rat pour l&#39;ARN ou de l&#39;extraction de protéines à partir région spécifique du cerveau

Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates ...

Homarus Americanus Stomatogastric Nervous System Dissection

With the goal of understanding how nervous systems produce activity and respond to the environment, neuroscientists turn to model systems that exhibit the activity of interest and are accessible and amenable to experimental methods. The stomatogastric nervous system (STNS) of the American lobster (Homarus americanus; also know was the Atlantic or Maine lobster) has been established as a model system for studying rhythm generating networks and neuromodulation of networks. The STNS consists of 3 anterior ganglia (2 commissural ganglia and an oesophageal ganglion), containing modulatory neurons that project centrally to the stomatogastric ganglion (STG). The STG contains approximately 30 neurons that comprise two central pattern generating networks, the pyloric and gastric networks that underlie feeding behaviors in crustaceans1,2. While it is possible to study this system in vivo3, the STNS continues to produce its rhythmic activity when isolated in vitro. Physical isolation of the STNS in a dish allows for easy access to the somata in the ganglia for intracellular electrophysiological recordings and to the nerves of the STNS for extracellular recordings. Isolating the STNS is a two-part process. The first part, dissecting the stomach from the animal, is described in an accompanying video article4. In this video article, fine dissection techniques are used to isolate the STNS from the stomach. This procedure results in a nervous system preparation that is available for electrophysiological recordings.

Homarus Americanus Stomatogastric Nervous System Dissection

We describe the fine dissection of the stomatogastric nervous system from the stomach of the American lobster (Homarus americanus).

Homarus americanus Stomatogastric dissection du système nerveux

With the goal of understanding how nervous systems produce activity and respond to the environment, neuroscientists turn to model systems that exhibit the ...

Gross and Fine Dissection of Inner Ear Sensory Epithelia in Adult Zebrafish (Danio rerio)

Neurosensory epithelia in the inner ear are the crucial structures for hearing and balance functions. Therefore, it is important to understand the cellular and molecular features of the epithelia, which are mainly composed of two types of cells: hair cells (HCs) and supporting cells (SCs). Here we choose to study the inner ear sensory epithelia in adult zebrafish not only because the epithelial structures are highly conserved in all vertebrates studied, but also because the adult zebrafish is able to regenerate HCs, an ability that mammals lose shortly after birth. We use the inner ear of adult zebrafish as a model system to study the mechanisms of inner ear HC regeneration in adult vertebrates that could be helpful for clinical therapy of hearing/balance deficits in human as a result of HC loss.
Here we demonstrate how to do gross and fine dissections of inner ear sensory epithelia in adult zebrafish. The gross dissection removes the tissues surrounding the inner ear and is helpful for preparing tissue sections, which allows us to examine the detailed structure of the sensory epithelia. The fine dissection cleans up the non-sensory-epithelial tissues of each individual epithelium and enables us to examine the heterogeneity of the whole epithelium easily in whole-mount epithelial samples.

Gross and Fine Dissection of Inner Ear Sensory Epithelia in Adult Zebrafish Danio rerio

The inner ear sensory epithelium of adult zebrafish is a good model system for understanding the mechanisms of hair cell regeneration in adult vertebrates. This protocol demonstrates the fine dissection of the epithelia, through which we can get tissue samples for studying the regenerative events at cellular and subcellular levels.

Dissection fine et globale de l&#39;épithélium sensoriel de l&#39;oreille interne adultes poisson zèbre ( Danio rerio)

Neurosensory epithelia in the inner ear are the crucial structures for hearing and balance functions. Therefore, it is important to understand the ...

Dissection of Organs from the Adult Zebrafish

Over the last 20 years, the zebrafish has become a powerful model organism for understanding vertebrate development and disease.  Although experimental analysis of the embryo and larva is extensive and the morphology has been well documented, descriptions of adult zebrafish anatomy and studies of development of the adult structures and organs, together with techniques for working with adults are lacking. The organs of the larva undergo significant changes in their overall structure, morphology, and anatomical location during the larval to adult transition.  Externally, the transparent larva develops its characteristic adult striped pigment pattern and paired pelvic fins, while internally, the organs undergo massive growth and remodeling.  In addition, the bipotential gonad primordium develops into either testis or ovary.  This protocol identifies many of the organs of the adult and demonstrates methods for dissection of the brain, gonads, gastrointestinal system, heart, and kidney of the adult zebrafish. The dissected organs can be used for in situ hybridization, immunohistochemistry, histology, RNA extraction, protein analysis, and other molecular techniques.  This protocol will assist in the broadening of studies in the zebrafish to include the remodeling of larval organs, the morphogenesis of organs specific to the adult and other investigations of the adult organ systems.

This protocol describes a procedure for identifying and dissecting organs from the adult zebrafish.

Dissection d&#39;organes sur le poisson zèbre pour adultes

Over the last 20 years, the zebrafish has become a powerful model organism for understanding vertebrate development and disease.  Although experimental ...

Using SCOPE to Identify Potential Regulatory Motifs in Coregulated Genes

SCOPE is an ensemble motif finder that uses three component algorithms in parallel to identify potential regulatory motifs by over-representation and motif position preference1. Each component algorithm is optimized to find a different kind of motif. By taking the best of these three approaches, SCOPE performs better than any single algorithm, even in the presence of noisy data1. In this article, we utilize a web version of SCOPE2 to examine genes that are involved in telomere maintenance. SCOPE has been incorporated into at least two other motif finding programs3,4 and has been used in other studies5-8. 
The three algorithms that comprise SCOPE are BEAM9, which finds non-degenerate motifs (ACCGGT), PRISM10, which finds degenerate motifs (ASCGWT), and SPACER11, which finds longer bipartite motifs (ACCnnnnnnnnGGT). These three algorithms have been optimized to find their corresponding type of motif. Together, they allow SCOPE to perform extremely well.
Once a gene set has been analyzed and candidate motifs identified, SCOPE can look for other genes that contain the motif which, when added to the original set, will improve the motif score. This can occur through over-representation or motif position preference. Working with partial gene sets that have biologically verified transcription factor binding sites, SCOPE was able to identify most of the rest of the genes also regulated by the given transcription factor.
Output from SCOPE shows candidate motifs, their significance, and other information both as a table and as a graphical motif map. FAQs and video tutorials are available at the SCOPE web site which also includes a "Sample Search" button that allows the user to perform a trial run. 
Scope has a very friendly user interface that enables novice users to access the algorithm's full power without having to become an expert in the bioinformatics of motif finding. As input, SCOPE can take a list of genes, or FASTA sequences. These can be entered in browser text fields, or read from a file. The output from SCOPE contains a list of all identified motifs with their scores, number of occurrences, fraction of genes containing the motif, and the algorithm used to identify the motif. For each motif, result details include a consensus representation of the motif, a sequence logo, a position weight matrix, and a list of instances for every motif occurrence (with exact positions and "strand" indicated). Results are returned in a browser window and also optionally by email. Previous papers describe the SCOPE algorithms in detail1,2,9-11.

A straight-forward and robust method to identify potential regulatory motifs in co-regulated genes is presented. SCOPE does not require any user parameters and returns motifs that represent excellent candidates for regulatory signals. The identification of such regulatory signals helps to understand the underlying biology.

Aide de la portée d&#39;identifier le potentiel Motifs de la réglementation dans les gènes co-régulés

SCOPE is an ensemble motif finder that uses three component algorithms in parallel to identify potential regulatory motifs by over-representation and ...

Dissection of the Adult Zebrafish Kidney

Researchers working in the burgeoning field of adult stem cell biology seek to understand the signals that regulate the behavior and function of stem cells during normal homeostasis and disease states. The understanding of adult stem cells has broad reaching implications for the future of regenerative medicine1. For example, better knowledge about adult stem cell biology can facilitate the design of therapeutic strategies in which organs are triggered to heal themselves or even the creation of methods for growing organs in vitro that can be transplanted into humans1. The zebrafish has become a powerful animal model for the study of vertebrate cell biology2. There has been extensive documentation and analysis of embryonic development in the zebrafish3. Only recently have scientists sought to document adult anatomy and surgical dissection techniques4, as there has been a progressive movement within the zebrafish community to broaden the applications of this research organism to adult studies. For example, there are expanding interests in using zebrafish to investigate the biology of adult stem cell populations and make sophisticated adult models of diseases such as cancer5. Historically, isolation of the zebrafish adult kidney has been instrumental for studying hematopoiesis, as the kidney is the anatomical location of blood cell production in fish6,7. The kidney is composed of nephron functional units found in arborized arrangements, surrounded by hematopoietic tissue that is dispersed throughout the intervening spaces. The hematopoietic component consists of hematopoietic stem cells (HSCs) and their progeny that inhabit the kidney until they terminally differentiate8. In addition, it is now appreciated that a group of renal stem/progenitor cells (RPCs) also inhabit the zebrafish kidney organ and enable both kidney regeneration and growth, as observed in other fish species9-11. In light of this new discovery, the zebrafish kidney is one organ that houses the location of two exciting opportunities for adult stem cell biology studies. It is clear that many outstanding questions could be well served with this experimental system. To encourage expansion of this field, it is beneficial to document detailed methods of visualizing and then isolating the adult zebrafish kidney organ. This protocol details our procedure for dissection of the adult kidney from both unfixed and fixed animals.
Dissection of the kidney organ can be used to isolate and characterize hematopoietic and renal stem cells and their offspring using established techniques such as histology, fluorescence activated cell sorting (FACS)11,12, expression profiling13,14, and transplantation11,15. 
We hope that dissemination of this protocol will provide researchers with the knowledge to implement broader use of zebrafish studies that ultimately can be translated for human application.

The zebrafish kidney is home to both renal and hematopoietic adult stem/progenitor cells, and represents an outstanding opportunity to study these cell types and their progeny in a vertebrate model organism. Here, we demonstrate a detailed dissection procedure that enables the researcher to identify and surgically remove the adult zebrafish kidney, which can be used for applications such as cell isolation, transplantation, and expression studies of kidney and/or blood cell populations.

Dissection du rein Zebrafish adultes

Researchers working in the burgeoning field of adult stem cell biology seek to understand the signals that regulate the behavior and function of stem ...

Dissection and Flat-mounting of the Threespine Stickleback Branchial Skeleton

The posterior pharyngeal segments of the vertebrate head give rise to the branchial skeleton, the primary site of food processing in fish. The morphology of the fish branchial skeleton is matched to a species' diet. Threespine stickleback fish (Gasterosteus aculeatus) have emerged as a model system to study the genetic and developmental basis of evolved differences in a variety of traits. Marine populations of sticklebacks have repeatedly colonized countless new freshwater lakes and creeks. Adaptation to the new diet in these freshwater environments likely underlies a series of craniofacial changes that have evolved repeatedly in independently derived freshwater populations. These include three major patterning changes to the branchial skeleton: reductions in the number and length of gill raker bones, increases in pharyngeal tooth number, and increased branchial bone lengths. Here we describe a detailed protocol to dissect and flat-mount the internal branchial skeleton in threespine stickleback fish. Dissection of the entire three-dimensional branchial skeleton and mounting it flat into a largely two-dimensional prep allows for the easy visualization and quantification of branchial skeleton morphology. This dissection method is inexpensive, fast, relatively easy, and applicable to a wide variety of fish species. In sticklebacks, this efficient method allows the quantification of skeletal morphology in genetic crosses to map genomic regions controlling craniofacial patterning.

The branchial skeleton, including gill rakers, pharyngeal teeth, and branchial bones, serves as the primary site of food processing in most fish. Here we describe a protocol to dissect and flat-mount this internal skeleton in threespine sticklebacks. This method is also applicable to a variety of other fish species.

Dissection et Flat-montage de l&#39;épinoche à trois épines branchiale Squelette

The posterior pharyngeal segments of the vertebrate head give rise to the branchial skeleton, the primary site of food processing in fish. The morphology ...

Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis

In most mammals, auditory ossicles in the middle ear, including the malleus, incus and stapes, are the smallest bones. In mice, a bony structure called the auditory bulla houses the ossicles, whereas the auditory capsule encloses the inner ear, namely the cochlea and semicircular canals. Murine ossicles are essential for hearing and thus of great interest to researchers in the field of otolaryngology, but their metabolism, development, and evolution are highly relevant to other fields. Altered bone metabolism can affect hearing function in adult mice, and various gene-deficient mice show changes in morphogenesis of auditory ossicles in utero. Although murine auditory ossicles are tiny, their manipulation is feasible if one understands their anatomical orientation and 3D structure. Here, we describe how to dissect the auditory bulla and capsule of postnatal mice and then isolate individual ossicles by removing part of the bulla. We also discuss how to embed the bulla and capsule in different orientations to generate paraffin or frozen sections suitable for preparation of longitudinal, horizontal, or frontal sections of the malleus. Finally, we enumerate anatomical differences between mouse and human auditory ossicles. These methods would be useful in analyzing pathological, developmental and evolutionary aspects of auditory ossicles and the middle ear in mice.

We present a protocol to isolate the auditory bulla, capsule, and ossicles from postnatal mice for whole mount and histological analysis.

Dissection de l&#39;Auditory Bulla chez les souris postnatale: Isolation des os de l&#39;oreille moyenne et l&#39;analyse histologique

In most mammals, auditory ossicles in the middle ear, including the malleus, incus and stapes, are the smallest bones. In mice, a bony structure called ...

Reverse Dissection and DiceCT Reveal Otherwise Hidden Data in the Evolution of the Primate Face

Facial expressions, or facial displays, of social or emotional intent are produced by many mammalian taxa as a means of visually communicating with conspecifics at a close range. These displays are achieved by contraction of the mimetic muscles, which are skeletal muscle attached to the dermis of the face. Reverse dissection, removing the full facial mask from the skull and approaching mimetic muscles in reverse, is an effective but destructive way of revealing the morphology of mimetic muscles but it is destructive. DiceCT is a novel mechanism for visualizing skeletal muscles, including mimetic muscles, and isolating individual muscle fascicles for quantitative measurement. Additionally, DiceCT provides a non-destructive mechanism for visualizing muscles. The combined techniques of reverse dissection and DiceCT can be used to assess the evolutionary morphology of mimetic musculature as well as potential contraction strength and velocity in these muscles. This study further demonstrates that DiceCT can be used to accurately and reliably visualize mimetic muscles as well as reverse dissection and provide a non-destructive method for sampling mimetic muscles.

Facial expressions are a mode of visual communication produced by mimetic muscles. Here, we present protocols for the novel techniques of reverse dissection and DiceCT to fully visualize and assess mimetic muscles. These combined techniques can examine both morphological and physiological aspects of mimetic musculature to determine functional aspects.

Inverser la Dissection et données DiceCT Reveal autrement cachés dans l’évolution du visage de primates

Facial expressions, or facial displays, of social or emotional intent are produced by many mammalian taxa as a means of visually communicating with ...

Dissection brut de l'estomac du homard, Homarus americanus

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