S'identifier

JoVE Journal

Bioengineering

This content is Open Access.

Operation of a Benchtop Bioreactor

87.7K Views

12:54 min

September 12th, 2013

September 12th, 2013

87,668 Views

0:05

Title

1:40

Fermentor Vessel Set-up

2:31

Fermentor Vessel Set-up: pH Probe Calibration

3:49

Fermentor Vessel Set-up: Continuation of Vessel Set-up and Calibration of pO2 Probe

8:01

Fermentor Vessel Set-up: Inoculation and Sampling

10:03

Results: A Typical Bioreactor Run

12:07

Conclusion

Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture’s biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.

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