October 11th, 2015
•Here, we present a protocol for specific siRNA-mediated mRNA depletion followed by immunofluorescence analysis to evaluate meiotic spindle assembly and organization in mouse oocytes. This protocol is suitable for in vitro depletion of transcripts and functional assessment of different spindle and/or MTOC-associated factors in oocytes.
Vidéos Connexes
Nuclear Transfer into Mouse Oocytes
Retrieval of Mouse Oocytes
Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos
Visualizing RNA Localization in Xenopus Oocytes
In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer
Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes
Flow Cytometry Purification of Mouse Meiotic Cells
Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms
Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy