Results: High Success Rate with Laser-assisted Cytoplasmic Microinjection
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Conclusion
Transcription
The overall goal of this procedure is to effectively deliver solutions into the cytoplasm of livestock zygotes using a blunt-end needle with the aid of a laser. This method is used in research and in the animal trans-genesis field, since one is as
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This protocol shows how to perform cytoplasmic microinjection in farm animal zygotes. This technique can be used to deliver any solution into the one-cell embryo such as genome editing tools to generate knockout animals.