Generation of Fluorescent Protein Cassettes by PCR
3:26
Transform Candida Cells with Fluorescent Protein DNA Cassette
7:31
Results: Generation of GFP and mCherry Fusions to Eno1
8:46
Conclusion
Transcription
The overall goal of this procedure is to generate fluorescent protein fusions in Candida species by using PCR mediated gene modification to tag a protein encoding sequence at its native genomic locus, thus ensuring stable integration and expressio
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PCR-mediated gene modification can be used to generate fluorescent protein fusions in Candida species, which facilitates visualization and quantitation of yeast cells and proteins. Herein, we present a strategy for constructing a fluorescent protein fusion (Eno1-FP) in Candida parapsilosis.