Bioluminescent EHEC Bacteria Preparation for Oral Inoculation
2:23
Mouse Oral Gavage of EHEC
3:27
Data Acquisition
4:46
Results: Detection of Enterohemorrhagic E. coli using In Vivo Bioluminescence
5:59
Conclusion
Transcription
The overall goal of this experiment is to detect enterohemorrhagic E.coli colonization in mice using a non-invasive in vivo imaging system. This method can help answer key questions in the enterohemorrhagic E.coli pathogenicity field such as colon
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A detailed protocol of a mouse model for enterohemorrhagic E. coli (EHEC) colonization by using bioluminescence-labeled bacteria is presented. The detection of these bioluminescent bacteria by a non-invasive in vivo imaging system in live animals can advance our current understanding of EHEC colonization.