Results: Visualization of Tangential Cell Migration
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Conclusion
Transcription
This method can help answer key questions in the developmental biology field, such as the mechanisms of neuronal cell migration. The main advantage of this technique is that you can detect individual and collective cell migration in the long term.
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We describe the methods for fluorescent labeling of tangentially migrating cells by electroporation, and for time-lapse imaging of the labeled cell movement in a flat-mount culture in order to visualize migrating cell behavior in the developing chick optic tectum.