Portable In Vitro Exposure Cassette (PIVEC) Assembly
3:45
Aerosol Exposure of Cells
4:52
Results: Deposition Efficiency and Expedited Post-exposure Analysis
6:00
Conclusion
Transcription
This protocol is significant, as the PIVEC allows for direct exposure of a cell culture to to real-world aerosols. The system can be warn to sample the same air a person breathes, enabling a new type of air quality monitoring. The main advantage o
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Here, we present a protocol to perform portable cellular aerosol exposures and measure cellular response. The method uses cells, grown at the air-liquid interface, mimicking in vivo physiology. Cellular response to copper nanoparticle aerosols was observed as oxidative stress through reactive oxygen species generation and cytotoxicity as lactate dehydrogenase release.